Hotspot

atpH-atpI

LSC · 12947-13704 · 758 bp

Back to Paronychia

Pi 0.0499

MarkerSeek score 80.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 758
variable_sites: 36
pi: 0.0499
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.0	57.9	829-845 bp	1.0	85.5
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.2	57.9	830-846 bp	1.0	84.3
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	ACCTGTCATGTTCCTGGGAT	45.0	50.0	58.0	58.3	877-893 bp	1.0	83.6
atpH-atpI_p4	TCCAGCAGCAATAACGGAAG	TTTTGCAACTTTAGCCGCAG	50.0	45.0	58.3	57.9	839-855 bp	1.0	83.6
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	TACCTGTCATGTTCCTGGGA	45.0	50.0	58.0	57.7	878-894 bp	1.0	83.5