Hotspot

rps16-trnQ-UUG

LSC · 5650-6333 · 684 bp

Back to Paronychia

Pi 0.0776

MarkerSeek score 86.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 684
variable_sites: 51
pi: 0.0776
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CCACAACGGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	778-807 bp	1.0	86.4
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	757-786 bp	1.0	86.4
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	768-797 bp	1.0	85.7
rps16-trnQ-UUG_p4	CCACAACGGATCATGTCCTT	TAAGGCATCGGGTTTTGGTC	50.0	50.0	57.9	58.2	808-837 bp	1.0	85.3
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	58.1	58.2	787-816 bp	1.0	85.3