Hotspot

rps15-ycf1

SSC · 121226-121542 · 317 bp

Back to Parnassia

Pi 0.0428

MarkerSeek score 71.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 317
variable_sites: 81
pi: 0.0428
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AGTTGTTCGGGTCCGTTTAC	TTTATCTCCTCGGCAATGCC	50.0	50.0	58.1	58.0	1304-1377 bp	1.0	90.7
rps15-ycf1_p2	GTTCGGGTCCGTTTACAGTT	TTTATCTCCTCGGCAATGCC	50.0	50.0	58.1	58.0	1300-1373 bp	1.0	90.7
rps15-ycf1_p3	CAGTTGTTCGGGTCCGTTTA	TTTATCTCCTCGGCAATGCC	50.0	50.0	58.1	58.0	1305-1378 bp	1.0	90.7
rps15-ycf1_p4	TTGTTCGGGTCCGTTTACAG	TTTATCTCCTCGGCAATGCC	50.0	50.0	58.1	58.0	1302-1375 bp	1.0	90.7
rps15-ycf1_p5	ATTTCCCAGTTGTTCGGGTC	TTTATCTCCTCGGCAATGCC	50.0	50.0	58.1	58.0	1311-1384 bp	0.923077	87.9