Hotspot

trnL-UAG-ccsA

SSC · 111737-111875 · 139 bp

Back to Parnassia

Pi 0.074

MarkerSeek score 74.20

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 139
variable_sites: 57
pi: 0.074
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	GTTAATATATGCTCGAAAGTCG	50.0	36.4	58.0	53.0	230-266 bp	1.0	52.0
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	GTTAATATATGCTCGAAAGTCG	50.0	36.4	58.0	53.0	229-265 bp	1.0	52.0
trnL-UAG-ccsA_p3	TAGACACGCTGCTCTTAGGA	GTTAATATATGCTCGAAAGTCG	50.0	36.4	57.9	53.0	220-256 bp	1.0	52.0
trnL-UAG-ccsA_p4	GGTAGACACGCTGCTCTTAG	GTTAATATATGCTCGAAAGTCG	55.0	36.4	57.8	53.0	222-258 bp	1.0	52.0
trnL-UAG-ccsA_p5	GTAGACACGCTGCTCTTAGG	GTTAATATATGCTCGAAAGTCG	55.0	36.4	57.8	53.0	221-257 bp	1.0	52.0