Hotspot

ndhF-rpl32

SSC · 110050-111001 · 952 bp

Back to Parnassia

Pi 0.0375

MarkerSeek score 78.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 952
variable_sites: 187
pi: 0.0375
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCTGTCGGAAAAAGGA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	1559-1882 bp	1.0	91.6
ndhF-rpl32_p2	TGTTGCTGTCGGAAAAAGGA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	1560-1883 bp	1.0	91.6
ndhF-rpl32_p3	CCTACAACAGGTCGCGTAAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	1975-2298 bp	1.0	91.4
ndhF-rpl32_p4	CCTACAACAGGTCGCGTAAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	1976-2299 bp	1.0	91.4
ndhF-rpl32_p5	TGTTGCTGTCGGAAAAAGGA	CGGCTTCCCTTGCTAGAAAT	45.0	50.0	58.0	58.0	1534-1836 bp	0.769231	82.1