Hotspot

rps11-rpl36

LSC · 78776-79053 · 278 bp

Back to Parnassia

Pi 0.045

MarkerSeek score 77.00

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 278
variable_sites: 63
pi: 0.045
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CTACGTGCATTCTTCCGTGA	ATCCGTAGACGAGGACGAAT	50.0	50.0	58.0	58.0	374-461 bp	0.961538	90.1
rps11-rpl36_p2	TCTACGTGCATTCTTCCGTG	ATCCGTAGACGAGGACGAAT	50.0	50.0	58.0	58.0	375-462 bp	0.961538	90.1
rps11-rpl36_p3	CTACGTGCATTCTTCCGTGA	GACTGATCCGTAGACGAGGA	50.0	55.0	58.0	58.0	379-466 bp	0.961538	90.0
rps11-rpl36_p4	TCTACGTGCATTCTTCCGTG	GACTGATCCGTAGACGAGGA	50.0	55.0	58.0	58.0	380-467 bp	0.961538	90.0
rps11-rpl36_p5	CTTCCGTGATCCGATACGTC	ATCCGTAGACGAGGACGAAT	55.0	50.0	58.0	58.0	363-450 bp	0.923077	88.4