Hotspot

petA-psbJ

LSC · 62241-62813 · 573 bp

Back to Parnassia

Pi 0.0375

MarkerSeek score 74.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 573
variable_sites: 90
pi: 0.0375
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGCATCGGTTATTTTGGCAC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.5	57.6	564-738 bp	1.0	85.4
petA-psbJ_p2	TCGCATCGGTTATTTTGGCA	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.5	57.6	565-739 bp	1.0	84.7
petA-psbJ_p3	TTCGCATCGGTTATTTTGGC	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.4	57.6	566-740 bp	1.0	84.7
petA-psbJ_p4	GCATCGGTTATTTTGGCACA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.4	57.6	563-737 bp	1.0	83.9
petA-psbJ_p5	CGCATCGGTTATTTTGGCAC	TATTCCGGATTGGGTTCATCC	50.0	47.6	58.5	57.5	565-739 bp	1.0	76.3