Hotspot

rps4-trnT-UGU

LSC · 46704-47377 · 674 bp

Back to Parnassia

Pi 0.0279

MarkerSeek score 75.90

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 674
variable_sites: 102
pi: 0.0279
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCCCAGACGGCGTATTTTT	TTTTAGCCCGCTTAGCTCAG	52.6	50.0	58.1	58.0	800-906 bp	1.0	82.7
rps4-trnT-UGU_p2	CCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	52.6	55.0	58.1	57.9	785-891 bp	1.0	81.8
rps4-trnT-UGU_p3	CCCCAGACGGCGTATTTTT	ATGGTCATCGGTTCGATTCC	52.6	50.0	58.1	57.8	753-859 bp	1.0	81.0
rps4-trnT-UGU_p4	CCCCAGACGGCGTATTTTT	CCGCTTAGCTCAGAGGTTAG	52.6	55.0	58.1	57.5	793-899 bp	1.0	78.9
rps4-trnT-UGU_p5	CCCCAGACGGCGTATTTTT	CAGAGGTTAGAGCATCGCAT	52.6	50.0	58.1	57.5	783-889 bp	1.0	78.7