Hotspot

trnG-UCC-trnR-UCU

LSC · 9060-9469 · 410 bp

Back to Parnassia

Pi 0.0524

MarkerSeek score 85.30

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 410
variable_sites: 116
pi: 0.0524
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AAATCAATTGCGTCCATGCG	50.0	45.0	58.0	58.1	523-583 bp	0.961538	89.5
trnG-UCC-trnR-UCU_p2	CAGCCTTCCAAGCTAACGAT	AAATCAATTGCGTCCATGCG	50.0	45.0	58.0	58.1	524-584 bp	0.961538	89.5
trnG-UCC-trnR-UCU_p3	CCTCAGCCTTCCAAGCTAAC	AAATCAATTGCGTCCATGCG	55.0	45.0	58.3	58.1	527-587 bp	0.961538	87.7
trnG-UCC-trnR-UCU_p4	CTAACGATGCGGGTTCGATT	AAATCAATTGCGTCCATGCG	50.0	45.0	58.4	58.1	512-572 bp	0.961538	86.3
trnG-UCC-trnR-UCU_p5	CGTCGACTATAACCCTCAGC	AAATCAATTGCGTCCATGCG	55.0	45.0	57.6	58.1	540-600 bp	0.923077	85.0