Hotspot

trnS-GCU-trnG-UCC

LSC · 7252-8167 · 916 bp

Back to Parnassia

Pi 0.0519
MarkerSeek score 85.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
916
variable_sites
221
pi
0.0519
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.98
markerseek_score
85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA GGGGCTATTAGAACGAATCACA 50.0 45.5 58.0 57.9 782-1077 bp 1.0 75.4
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC GGGGCTATTAGAACGAATCACA 50.0 45.5 58.0 57.9 781-1076 bp 1.0 75.4
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA AGGGGCTATTAGAACGAATCAC 50.0 45.5 58.0 57.7 783-1078 bp 1.0 73.3
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC AGGGGCTATTAGAACGAATCAC 50.0 45.5 58.0 57.7 782-1077 bp 1.0 73.2
trnS-GCU-trnG-UCC_p5 AGCAATCCGACGCTTTAGTC GGGGCTATTAGAACGAATCACA 50.0 45.5 58.4 57.9 784-1079 bp 1.0 72.6