Hotspot

psbK-psbI

LSC · 6510-6878 · 369 bp

Back to Parnassia

Pi 0.0386

MarkerSeek score 74.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 369
variable_sites: 73
pi: 0.0386
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	AGGAATCCGAAGATGAATAGAGA	50.0	39.1	57.9	56.3	300-500 bp	0.923077	51.2
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	AGGAATCCGAAGATGAATAGAGA	45.0	39.1	58.3	56.3	297-497 bp	0.923077	49.2
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	AGGAATCCGAAGATGAATAGAGA	45.0	39.1	58.3	56.3	298-498 bp	0.923077	49.2
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	AGGAATCCGAAGATGAATAGAGA	45.0	39.1	58.3	56.3	299-499 bp	0.923077	49.2
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	AGGAATCCGAAGATGAATAGAGAA	50.0	37.5	57.9	57.0	300-500 bp	0.923077	48.9