Hotspot

trnK-UUU-rps16

LSC · 4311-5091 · 781 bp

Back to Parnassia

Pi 0.0385

MarkerSeek score 74.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 148
pi: 0.0385
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	GGTTTTGCGCCAAGAATTCA	TTTCCTTGAAAGGGGTGCTC	45.0	50.0	57.9	58.0	833-1444 bp	1.0	90.8
trnK-UUU-rps16_p2	TGGTTTTGCGCCAAGAATTC	TTTCCTTGAAAGGGGTGCTC	45.0	50.0	57.9	58.0	834-1445 bp	1.0	90.8
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.2	58.0	632-1243 bp	1.0	90.6
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.2	58.0	631-1242 bp	1.0	90.6
trnK-UUU-rps16_p5	GGTTTTGCGCCAAGAATTCA	ATCCCGAAGAGATGGCAAAC	45.0	50.0	57.9	58.0	923-1534 bp	1.0	90.5