Hotspot

ndhA

SSC · 124623-127060 · 2438 bp

Back to Parkinsonia

Pi 0.0192

MarkerSeek score 69.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2438
variable_sites: 46
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAAGGAACA	CCCCCGGTTTTATCAATTTGC	42.9	47.6	56.3	58.1	2616-2656 bp	1.0	52.0
ndhA_p2	AACCCAGTTACCATAAGGAACA	CCCCCGGTTTTATCAATTTGC	40.9	47.6	57.0	58.1	2617-2657 bp	1.0	50.0
ndhA_p3	TGAACCCAGTTACCATAAGGA	CCCCCGGTTTTATCAATTTGC	42.9	47.6	56.0	58.1	2619-2659 bp	1.0	49.8
ndhA_p4	GAACCCAGTTACCATAAGGAACA	CCCCCGGTTTTATCAATTTGC	43.5	47.6	58.1	58.1	2618-2658 bp	1.0	49.3
ndhA_p5	TGAACCCAGTTACCATAAGGAAC	CCCCCGGTTTTATCAATTTGC	43.5	47.6	58.1	58.1	2619-2659 bp	1.0	49.3