Hotspot

ndhG-ndhI

SSC · 123664-124057 · 394 bp

Back to Parkinsonia

Pi 0.0705

MarkerSeek score 89.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 394
variable_sites: 26
pi: 0.0705
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 89.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCCAACGAAAAGGCAGAAA	AATCAAATTGCTTTGGGCCG	45.0	45.0	57.9	57.9	589-596 bp	1.0	85.6
ndhG-ndhI_p2	TCCCAACGAAAAGGCAGAAA	TTTGGGCCGTTTACCAATGT	45.0	45.0	57.9	58.3	578-585 bp	1.0	84.2
ndhG-ndhI_p3	TCCCAACGAAAAGGCAGAAA	CTTTGGGCCGTTTACCAATG	45.0	50.0	57.9	57.6	579-586 bp	1.0	83.6
ndhG-ndhI_p4	TCCCAACGAAAAGGCAGAAA	CAAATTGCTTTGGGCCGTTT	45.0	45.0	57.9	58.4	586-593 bp	1.0	83.2
ndhG-ndhI_p5	TCCCAACGAAAAGGCAGAAA	AATTGCTTTGGGCCGTTTAC	45.0	45.0	57.9	57.6	584-591 bp	1.0	82.9