Hotspot

ndhD-psaC

SSC · 121973-122078 · 106 bp

Back to Parkinsonia

Pi 0.123

MarkerSeek score 76.80

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 106
variable_sites: 13
pi: 0.123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGAAGATTAAGGAACCCGCG	AAACAACTCGAAGCATGGGT	50.0	45.0	57.7	58.0	232 bp	1.0	81.8
ndhD-psaC_p2	AGAAGATTAAGGAACCCGCG	GGCATGAAACAACTCGAAGC	50.0	50.0	57.7	58.0	238 bp	1.0	81.6
ndhD-psaC_p3	TAAGGAACCCGCGGATATTG	AAACAACTCGAAGCATGGGT	50.0	45.0	57.5	58.0	225 bp	1.0	80.2
ndhD-psaC_p4	TAAGGAACCCGCGGATATTG	GGCATGAAACAACTCGAAGC	50.0	50.0	57.5	58.0	231 bp	1.0	80.0
ndhD-psaC_p5	GAAGATTAAGGAACCCGCGG	AAACAACTCGAAGCATGGGT	55.0	45.0	58.7	58.0	231 bp	1.0	78.7