Hotspot

rps8-rpl14

LSC · 85563-85947 · 385 bp

Back to Parkinsonia

Pi 0.0696

MarkerSeek score 76.30

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 385
variable_sites: 24
pi: 0.0696
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GCAATGGTGTCCTTACCCAT	GTTTCATTAGCACCCGAGGT	50.0	50.0	57.9	57.9	428-432 bp	1.0	86.4
rps8-rpl14_p2	GATGGTATTCGAACCGTCCC	GTTTCATTAGCACCCGAGGT	55.0	50.0	58.1	57.9	491-495 bp	1.0	85.9
rps8-rpl14_p3	GCAATGGTGTCCTTACCCAT	AGTTTCATTAGCACCCGAGG	50.0	50.0	57.9	57.6	429-433 bp	1.0	84.1
rps8-rpl14_p4	GGTATTCGAACCGTCCCTTT	GTTTCATTAGCACCCGAGGT	50.0	50.0	57.6	57.9	488-492 bp	1.0	83.9
rps8-rpl14_p5	GATGGTATTCGAACCGTCCC	AGTTTCATTAGCACCCGAGG	55.0	50.0	58.1	57.6	492-496 bp	1.0	83.6