Hotspot

rps11-rpl36

LSC · 84210-84560 · 351 bp

Back to Parkinsonia

Pi 0.0523

MarkerSeek score 75.30

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 351
variable_sites: 17
pi: 0.0523
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TACGCGCATTCTTACGTGAA	TCCAAAACATAAACAAAGACAAGGA	45.0	32.0	58.0	57.6	449-521 bp	1.0	46.8
rps11-rpl36_p2	TTACGCGCATTCTTACGTGA	TCCAAAACATAAACAAAGACAAGGA	45.0	32.0	58.0	57.6	450-522 bp	1.0	46.8
rps11-rpl36_p3	TACGCGCATTCTTACGTGAA	CCAAAACATAAACAAAGACAAGGA	45.0	33.3	58.0	56.3	448-520 bp	1.0	46.8
rps11-rpl36_p4	TACGCGCATTCTTACGTGAA	TCCAAAACATAAACAAAGACAAGG	45.0	33.3	58.0	56.3	449-521 bp	1.0	46.8
rps11-rpl36_p5	CCCTTTGGTATTTTACGCGC	TCCAAAACATAAACAAAGACAAGGA	50.0	32.0	57.8	57.6	462-534 bp	1.0	46.7