Hotspot

rbcL-accD

LSC · 60832-61893 · 1062 bp

Back to Parkinsonia

Pi 0.0619

MarkerSeek score 85.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1062
variable_sites: 61
pi: 0.0619
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGTCCTGAATTAGCTGCTGC	ACTTAGCCCACACCTGTATTC	50.0	47.6	58.0	57.4	1185-1203 bp	1.0	74.8
rbcL-accD_p2	AGTCCTGAATTAGCTGCTGC	AGCCCACACCTGTATTCTAAC	50.0	47.6	58.0	57.4	1181-1199 bp	1.0	74.8
rbcL-accD_p3	AGTCCTGAATTAGCTGCTGC	GCCCACACCTGTATTCTAACT	50.0	47.6	58.0	57.4	1180-1198 bp	1.0	74.8
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	AGCCCACACCTGTATTCTAAC	55.0	47.6	58.0	57.4	1196-1214 bp	1.0	74.7
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	GCCCACACCTGTATTCTAACT	55.0	47.6	58.0	57.4	1195-1213 bp	1.0	74.7