Hotspot

ndhC-trnV-UAC

LSC · 54809-55534 · 726 bp

Back to Parkinsonia

Pi 0.0828

MarkerSeek score 82.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 726
variable_sites: 56
pi: 0.0828
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCAATGAAGCAGCAACTGGT	AAAAGATGCACGTTGGGTCT	45.0	45.0	58.0	58.0	1374-1484 bp	1.0	86.1
ndhC-trnV-UAC_p2	AGAGAAGCGGCCATAAACTG	AAAAGATGCACGTTGGGTCT	50.0	45.0	58.0	58.0	1349-1459 bp	1.0	86.1
ndhC-trnV-UAC_p3	TCAATGAAGCAGCAACTGGT	TGCACGTTGGGTCTGTAAAA	45.0	45.0	58.0	58.0	1368-1478 bp	1.0	85.9
ndhC-trnV-UAC_p4	CCGGTAATTGTACAGGCTCC	AAAAGATGCACGTTGGGTCT	55.0	45.0	58.1	58.0	1569-1679 bp	1.0	85.1
ndhC-trnV-UAC_p5	CGGTAATTGTACAGGCTCCC	AAAAGATGCACGTTGGGTCT	55.0	45.0	58.1	58.0	1568-1678 bp	1.0	85.1