Hotspot

trnT-UGU-trnL-UAA

LSC · 50035-51299 · 1265 bp

Back to Parkinsonia

Pi 0.0344

MarkerSeek score 76.80

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1265
variable_sites: 41
pi: 0.0344
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	GCGTCTACCTATTTCGCCAT	55.0	50.0	57.9	57.8	1321-1331 bp	1.0	84.2
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.8	57.8	1353-1363 bp	1.0	83.3
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	1358-1368 bp	1.0	81.3
trnT-UGU-trnL-UAA_p4	CTAACCTCTGAGCTAAGCGG	GCGTCTACCTATTTCGCCAT	55.0	50.0	57.5	57.8	1313-1323 bp	1.0	81.3
trnT-UGU-trnL-UAA_p5	ATGCGATGCTCTAACCTCTG	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.5	57.8	1323-1333 bp	1.0	81.1