Hotspot

psbC-trnS-UGA

LSC · 38960-39196 · 237 bp

Back to Parkinsonia

Pi 0.0638

MarkerSeek score 78.00

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 237
variable_sites: 15
pi: 0.0638
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	AGCTGCAGCAGGATTTGAAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.4	58.0	400-402 bp	1.0	80.2
psbC-trnS-UGA_p2	AGCTGCAGCAGGATTTGAAA	GCTGAGTGGTTGATAGCTCC	45.0	55.0	58.4	58.1	392-394 bp	1.0	80.0
psbC-trnS-UGA_p3	AGCTGCAGCAGGATTTGAAA	GGCTGAGTGGTTGATAGCTC	45.0	55.0	58.4	58.1	393-395 bp	1.0	80.0
psbC-trnS-UGA_p4	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	385-387 bp	1.0	80.0
psbC-trnS-UGA_p5	TAGGTCATTTATGGCACGCG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.4	58.0	434-436 bp	1.0	79.6