Hotspot

trnS-GCU-trnG-UCC

LSC · 9150-9977 · 828 bp

Back to Parkinsonia

Pi 0.072

MarkerSeek score 79.80

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 828
variable_sites: 50
pi: 0.072
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	GGATCCATAGAACGAATCGCA	50.0	47.6	58.0	58.0	796-908 bp	1.0	80.4
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	GGATCCATAGAACGAATCGCA	50.0	47.6	58.0	58.0	795-907 bp	1.0	80.4
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	GGATCCATAGAACGAATCGCA	50.0	47.6	58.2	58.0	850-962 bp	1.0	79.0
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.0	58.2	785-897 bp	1.0	78.8
trnS-GCU-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	ACGAATCGCACTTTTACCACT	50.0	42.9	58.0	58.2	784-896 bp	1.0	78.8