Hotspot

trnQ-UUG-psbK

LSC · 7600-8144 · 545 bp

Back to Parkinsonia

Pi 0.062

MarkerSeek score 80.90

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 545
variable_sites: 33
pi: 0.062
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GACCAAAACCCGTTGCCTTA	AGGCTTCAGGTAATTTCGCG	50.0	50.0	58.7	58.6	671-714 bp	1.0	74.8
trnQ-UUG-psbK_p2	GACCAAAACCCGTTGCCTTA	GGCTTCAGGTAATTTCGCGA	50.0	50.0	58.7	58.6	670-713 bp	1.0	74.8
trnQ-UUG-psbK_p3	GAATAGCGGGACCAAAACCC	AGGCTTCAGGTAATTTCGCG	55.0	50.0	58.9	58.6	680-723 bp	1.0	73.0
trnQ-UUG-psbK_p4	GAATAGCGGGACCAAAACCC	GGCTTCAGGTAATTTCGCGA	55.0	50.0	58.9	58.6	679-722 bp	1.0	73.0
trnQ-UUG-psbK_p5	ATATGCTCTGGGACGGAAGG	AGGCTTCAGGTAATTTCGCG	55.0	50.0	58.9	58.6	712-755 bp	1.0	72.4