Hotspot

rps16-trnQ-UUG

LSC · 6846-7527 · 682 bp

Back to Parkinsonia

Pi 0.0481

MarkerSeek score 87.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 682
variable_sites: 32
pi: 0.0481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 87.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	762-856 bp	1.0	84.2
rps16-trnQ-UUG_p2	ATGTCTTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	764-858 bp	1.0	83.2
rps16-trnQ-UUG_p3	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	750-844 bp	1.0	82.7
rps16-trnQ-UUG_p4	CATGTCTTTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	765-859 bp	1.0	82.5
rps16-trnQ-UUG_p5	TGTCTTTCAAGTCGCACGTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.4	58.1	763-857 bp	1.0	81.4