Hotspot

trnH-GUG-psbA

LSC · 83-471 · 389 bp

Back to Parkinsonia

Pi 0.0656

MarkerSeek score 82.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 389
variable_sites: 24
pi: 0.0656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	435-458 bp	1.0	86.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	449-472 bp	1.0	86.5
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	448-471 bp	1.0	86.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	450-473 bp	1.0	86.5
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CCGAGCTAACCTTGGTATGG	55.0	55.0	58.1	57.8	484-507 bp	1.0	84.6