Hotspot

ndhE

SSC · 124555-124860 · 306 bp

Back to Parkia

Pi NA
MarkerSeek score 28.20
Primer available yes
Label ndhE

Feature Metadata

label_name
ndhE
feature_type
gene
region
SSC
length_bp
306
variable_sites
0
pi
NA
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.00
markerseek_score
28.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE_p1 CGTTTTCGCAAACATGATCA CGAAATTGATAAGGAGTTGGTCA 40.0 39.1 55.6 56.9 428 bp 1.0 44.0
ndhE_p2 TCGCAAACATGATCATACGT CGAAATTGATAAGGAGTTGGTCA 40.0 39.1 55.6 56.9 423 bp 1.0 44.0
ndhE_p3 CGTTTTCGCAAACATGATCA TCGAAATTGATAAGGAGTTGGTCA 40.0 37.5 55.6 58.2 429 bp 1.0 44.0
ndhE_p4 TCGCAAACATGATCATACGT TCGAAATTGATAAGGAGTTGGTCA 40.0 37.5 55.6 58.2 424 bp 1.0 44.0
ndhE_p5 TCGCAAACATGATCATACGTT CGAAATTGATAAGGAGTTGGTCA 38.1 39.1 56.4 56.9 423 bp 1.0 44.0