Hotspot

rps3-rps19

LSC · 90490-90915 · 426 bp

Back to Parkia

Pi 0.0266
MarkerSeek score 73.00
Primer available yes
Label rps3-rps19

Feature Metadata

label_name
rps3-rps19
feature_type
igs
region
LSC
length_bp
426
variable_sites
11
pi
0.0266
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rps19_p1 TACCAAGTCTGAAACCGAGC ATAAACTTCCGGGGACATGC 50.0 50.0 57.5 57.9 512-514 bp 1.0 79.6
rps3-rps19_p2 GTCTGAAACCGAGCGGATTT ATAAACTTCCGGGGACATGC 50.0 50.0 58.6 57.9 506-508 bp 1.0 78.7
rps3-rps19_p3 TGGTTGTGCAAACCAAAGAG ATAAACTTCCGGGGACATGC 45.0 50.0 57.0 57.9 550-552 bp 1.0 75.3
rps3-rps19_p4 TCTGAAACCGAGCGGATTTA ATAAACTTCCGGGGACATGC 45.0 50.0 57.0 57.9 505-507 bp 1.0 74.9
rps3-rps19_p5 GTCTGAAACCGAGCGGATT ATAAACTTCCGGGGACATGC 52.6 50.0 57.9 57.9 506-508 bp 1.0 74.1