Hotspot

rpl33-rps18

LSC · 74116-74532 · 417 bp

Back to Parkia

Pi 0.00822
MarkerSeek score 62.30
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
417
variable_sites
3
pi
0.00822
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 TCTGTCCCTGTTGTTGCAAA TCAATTCGATCCCCCGATTG 45.0 50.0 57.9 57.7 507-553 bp 1.0 81.3
rpl33-rps18_p2 TTCTGTCCCTGTTGTTGCAA TCAATTCGATCCCCCGATTG 45.0 50.0 57.9 57.7 508-554 bp 1.0 81.3
rpl33-rps18_p3 ATTCTGTCCCTGTTGTTGCA TCAATTCGATCCCCCGATTG 45.0 50.0 57.6 57.7 509-555 bp 1.0 79.5
rpl33-rps18_p4 TCTGTCCCTGTTGTTGCAAA GGGCAAACGTCTACGAAAAG 45.0 50.0 57.9 57.4 481-527 bp 1.0 78.7
rpl33-rps18_p5 TTCTGTCCCTGTTGTTGCAA GGGCAAACGTCTACGAAAAG 45.0 50.0 57.9 57.4 482-528 bp 1.0 78.7