Hotspot

petA-psbJ

LSC · 68801-70083 · 1283 bp

Back to Parkia

Pi 0.0125
MarkerSeek score 54.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1283
variable_sites
16
pi
0.0125
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 AGAAACAGTTTGAGAAGGTTCA ATTCCGGATTGGGTTCATCC 36.4 50.0 55.8 57.6 1353-1389 bp 1.0 44.3
petA-psbJ_p2 AAGAAACAGTTTGAGAAGGTTCA ATTCCGGATTGGGTTCATCC 34.8 50.0 56.5 57.6 1354-1390 bp 1.0 42.1
petA-psbJ_p3 AGAAACAGTTTGAGAAGGTTCAA ATTCCGGATTGGGTTCATCC 34.8 50.0 56.5 57.6 1353-1389 bp 1.0 42.1
petA-psbJ_p4 GAAACAGTTTGAGAAGGTTCA ATTCCGGATTGGGTTCATCC 38.1 50.0 54.3 57.6 1352-1388 bp 1.0 40.9
petA-psbJ_p5 AAGAAACAGTTTGAGAAGGTTCAA ATTCCGGATTGGGTTCATCC 33.3 50.0 57.2 57.6 1354-1390 bp 1.0 40.9