Hotspot

trnT-UGU-trnL-UAA

LSC · 51716-52902 · 1187 bp

Back to Parkia

Pi 0.00621
MarkerSeek score 67.10
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1187
variable_sites
7
pi
0.00621
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 1259-1269 bp 1.0 82.2
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 1267-1277 bp 1.0 81.7
trnT-UGU-trnL-UAA_p3 AAATGCGACGCTCTAACCTC ACCAATTTCGCCATATCCCC 50.0 50.0 58.4 58.0 1231-1241 bp 1.0 81.4
trnT-UGU-trnL-UAA_p4 AAATGCGACGCTCTAACCTC TAGCGTCTACCAATTTCGCC 50.0 50.0 58.4 58.1 1239-1249 bp 1.0 80.8
trnT-UGU-trnL-UAA_p5 GGAATCGAACCGATGACCAT AGTCCGTAGCGTCTACCAAT 50.0 50.0 57.8 58.2 1273-1283 bp 1.0 80.3