Hotspot

psbZ-trnG-GCC

LSC · 40895-41826 · 932 bp

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Pi 0.0129
MarkerSeek score 67.40
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
932
variable_sites
10
pi
0.0129
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGTCG GCGTCTTCTCTTTGGCAAAG 55.0 50.0 58.0 57.7 931-1087 bp 1.0 82.1
psbZ-trnG-GCC_p2 CTTCTCCTGATGGTTGGTCG ATACATAAATAGGGGGCGCG 55.0 50.0 58.0 57.6 975-1131 bp 1.0 81.5
psbZ-trnG-GCC_p3 TTGCTTCTCCTGATGGTTGG GCGTCTTCTCTTTGGCAAAG 50.0 50.0 57.8 57.7 934-1090 bp 1.0 80.6
psbZ-trnG-GCC_p4 TCCTGATGGTTGGTCGAGTA GCGTCTTCTCTTTGGCAAAG 50.0 50.0 57.8 57.7 927-1083 bp 1.0 80.4
psbZ-trnG-GCC_p5 GCTTCTCCTGATGGTTGGTC GCGTCTTCTCTTTGGCAAAG 55.0 50.0 58.3 57.7 932-1088 bp 1.0 80.2