Hotspot

trnC-GCA-petN

LSC · 30881-32179 · 1299 bp

Back to Parkia

Pi 0.00394
MarkerSeek score 63.20
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
1299
variable_sites
5
pi
0.00394
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 1355-1359 bp 1.0 80.1
trnC-GCA-petN_p2 GTATCATTTTGGCGGCATGG AGCCCAAGCGAGACTTACTA 50.0 50.0 57.9 58.2 1407-1411 bp 1.0 79.6
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 1416-1420 bp 1.0 78.5
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG TCTTCCCCATACTACGAGGG 50.0 55.0 58.1 57.6 1406-1410 bp 1.0 78.3
trnC-GCA-petN_p5 GTATCATTTTGGCGGCATGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 57.9 57.6 1468-1472 bp 1.0 77.9