Hotspot

atpH-atpI

LSC · 15793-17241 · 1449 bp

Back to Parkia

Pi 0.00972
MarkerSeek score 64.90
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1449
variable_sites
14
pi
0.00972
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 CCTTCTACAGCTTGACCTGC TTTTGCAACTTTAGCTGCGG 55.0 45.0 58.3 57.9 1617-1627 bp 1.0 77.9
atpH-atpI_p2 TCCCTTCTACAGCTTGACCT TTTTGCAACTTTAGCTGCGG 50.0 45.0 57.7 57.9 1619-1629 bp 1.0 77.5
atpH-atpI_p3 CCCTTCTACAGCTTGACCTG TTTTGCAACTTTAGCTGCGG 55.0 45.0 57.6 57.9 1618-1628 bp 1.0 76.9
atpH-atpI_p4 GCGATCCCTTCTACAGCTTG TTTTGCAACTTTAGCTGCGG 55.0 45.0 58.4 57.9 1623-1633 bp 1.0 76.7
atpH-atpI_p5 AGGTCCAATAGAAGCAAGCC TTTTGCAACTTTAGCTGCGG 50.0 45.0 57.6 57.9 1576-1586 bp 1.0 76.7