Hotspot

trnR-UCU-atpA

LSC · 11569-12027 · 459 bp

Back to Parkia

Pi 0.0155
MarkerSeek score 66.20
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
459
variable_sites
7
pi
0.0155
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC CATTCACTCGGGAAGCAGAA 47.6 50.0 57.3 57.9 573-595 bp 1.0 67.6
trnR-UCU-atpA_p2 GGTTCAAATCCTATTGGACGC AAGACATTCACTCGGGAAGC 47.6 50.0 57.3 57.9 577-599 bp 1.0 67.6
trnR-UCU-atpA_p3 GGTTCAAATCCTATTGGACGC GGAAGCAGAAGCCCTTTTGA 47.6 50.0 57.3 58.4 563-585 bp 1.0 65.4
trnR-UCU-atpA_p4 GGTTCAAATCCTATTGGACGC ATTCACTCGGGAAGCAGAAG 47.6 50.0 57.3 57.6 572-594 bp 1.0 65.2
trnR-UCU-atpA_p5 TGGATAGGACAGAGGTCTTCT CATTCACTCGGGAAGCAGAA 47.6 50.0 56.9 57.9 608-630 bp 1.0 64.6