Hotspot

trnK-UUU-rps16

LSC · 4448-5929 · 1482 bp

Back to Parkia

Pi 0.0348
MarkerSeek score 65.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1482
variable_sites
43
pi
0.0348
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 GGGCCCGATCCAATAACAAT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.0 58.0 1667-1796 bp 1.0 85.8
trnK-UUU-rps16_p2 GGGCCCGATCCAATAACAAT TGAGGTTGCGAATACGAGTG 50.0 50.0 58.0 58.0 2019-2148 bp 1.0 85.7
trnK-UUU-rps16_p3 CCTTTCTGGATCAGTCGTGG TGAGGTTGCGAATACGAGTG 55.0 50.0 58.0 58.0 1946-2075 bp 1.0 85.6
trnK-UUU-rps16_p4 GGGCCCGATCCAATAACAAT AAGCTAACGGATCGTGGAAC 50.0 50.0 58.0 58.0 2210-2339 bp 1.0 85.5
trnK-UUU-rps16_p5 CCTTTCTGGATCAGTCGTGG AAGCTAACGGATCGTGGAAC 55.0 50.0 58.0 58.0 2137-2266 bp 1.0 85.5