Hotspot

ycf2

IRa · 146638-153543 · 6906 bp

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Pi 0.00485
MarkerSeek score 42.40
Primer available yes
Label ycf2

Feature Metadata

label_name
ycf2
feature_type
gene
region
IRa
length_bp
6906
variable_sites
238
pi
0.00485
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2_p1 TCAGCCAATTGTCTCGATCG TCGAACCGAGAGATCCATGA 50.0 50.0 58.1 57.9 743-806 bp 1.0 89.5
ycf2_p2 TCAGCCAATTGTCTCGATCG GGGGATACACGTTACACCAC 50.0 55.0 58.1 58.0 1176-1392 bp 1.0 88.4
ycf2_p3 AAGCCTCGGACCAATCAATC GGGGATACACGTTACACCAC 50.0 55.0 58.0 58.0 705-882 bp 0.897436 81.7
ycf2_p4 AAGCCTCGGACCAATCAATC TCGAACCGAGAGATCCATGA 50.0 50.0 58.0 57.9 278-296 bp 0.897436 81.3
ycf2_p5 AAGAAACGCAAACGAAGTGC GGGGATACACGTTACACCAC 45.0 55.0 58.2 58.0 748-925 bp 0.897436 80.5