Hotspot

ycf2-trnI_CAU

IRa · 153544-153739 · 196 bp

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Pi 0.0499
MarkerSeek score 65.60
Primer available yes
Label ycf2-trnI_CAU

Feature Metadata

label_name
ycf2-trnI_CAU
feature_type
igs
region
IRa
length_bp
196
variable_sites
71
pi
0.0499
unique_haplotype_count
20
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnI_CAU_p1 AGGATTTTAATTGATGTCGTTTCA GTTGGGCGCTTTAACCATTC 29.2 50.0 55.4 58.0 203-628 bp 0.846154 40.4
ycf2-trnI_CAU_p2 AGGATTTTAATTGATGTCGTTTCA TTGGGCGCTTTAACCATTCA 29.2 45.0 55.4 58.4 202-627 bp 0.846154 40.4
ycf2-trnI_CAU_p3 GGATTTTAATTGATGTCGTTTCA GTTGGGCGCTTTAACCATTC 30.4 50.0 54.0 58.0 202-627 bp 0.846154 40.4
ycf2-trnI_CAU_p4 AGGATTTTAATTGATGTCGTTTCA AGTTGGGCGCTTTAACCATT 29.2 45.0 55.4 58.4 204-629 bp 0.846154 40.3
ycf2-trnI_CAU_p5 AGGATTTTAATTGATGTCGTTTCA ATTGAACATTGGCGTGCATC 29.2 45.0 55.4 57.7 259-684 bp 0.846154 39.9