Hotspot

ndhF-rpl32

SSC · 113373-114147 · 775 bp

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Pi 0.0134
MarkerSeek score 59.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
775
variable_sites
61
pi
0.0134
unique_haplotype_count
29
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGCATATTGATATGTATGTTCCA AGAAAGAGCTTTTACTGCAGT 30.4 38.1 53.7 55.4 849-974 bp 1.0 49.5
ndhF-rpl32_p2 TGCATATTGATATGTATGTTCCA AAGAAAGAGCTTTTACTGCAGT 30.4 36.4 53.7 56.2 850-975 bp 1.0 49.5
ndhF-rpl32_p3 TGCATATTGATATGTATGTTCCA AGAAAGAGCTTTTACTGCAGTT 30.4 36.4 53.7 56.2 849-974 bp 1.0 49.5
ndhF-rpl32_p4 ATGCATATTGATATGTATGTTCCA AGAAAGAGCTTTTACTGCAGT 29.2 38.1 54.3 55.4 850-975 bp 1.0 49.5
ndhF-rpl32_p5 TGCATATTGATATGTATGTTCCAT AGAAAGAGCTTTTACTGCAGT 29.2 38.1 54.3 55.4 849-974 bp 1.0 49.5