Hotspot

ndhD

SSC · 122465-123967 · 1503 bp

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Pi 0.00244

MarkerSeek score 46.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 7
pi: 0.00244
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AAAAGGGGGCAAAGGCATAA	AACAAATTGCCTCTGCTCCA	45.0	45.0	58.0	58.0	1925-1926 bp	1.0	80.2
ndhD_p2	AAAAGGGGGCAAAGGCATAA	ACAAATTGCCTCTGCTCCAA	45.0	45.0	58.0	58.0	1924-1925 bp	1.0	80.2
ndhD_p3	AAAGGGGGCAAAGGCATAAA	AACAAATTGCCTCTGCTCCA	45.0	45.0	58.0	58.0	1924-1925 bp	1.0	80.2
ndhD_p4	AAAGGGGGCAAAGGCATAAA	ACAAATTGCCTCTGCTCCAA	45.0	45.0	58.0	58.0	1923-1924 bp	1.0	80.2
ndhD_p5	AAAGGGGGCAAAGGCATAAA	ATACCTTGGGACGGGTGTAA	45.0	50.0	58.0	58.0	1949-1950 bp	1.0	79.9