Hotspot

atpA

LSC · 10295-11827 · 1533 bp

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Pi 0.000978

MarkerSeek score 36.90

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1533
variable_sites: 3
pi: 0.000978
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	TGGAAAAGCGTCCATTGTCT	GAACTACGCAATGGGACCAT	45.0	50.0	57.7	58.0	2309-2394 bp	1.0	81.0
atpA_p2	TGGAAAAGCGTCCATTGTCT	AGAGTCCTCCGAGTATTCCG	45.0	55.0	57.7	58.0	2612-2697 bp	1.0	80.6
atpA_p3	TGGAAAAGCGTCCATTGTCT	GAGTCCTCCGAGTATTCCGA	45.0	55.0	57.7	58.0	2611-2696 bp	1.0	80.6
atpA_p4	TGGAAAAGCGTCCATTGTCT	AAGAACTACGCAATGGGACC	45.0	50.0	57.7	57.9	2311-2396 bp	1.0	80.4
atpA_p5	TGGAAAAGCGTCCATTGTCT	TCATGTTTGGTTCGGGAAGG	45.0	50.0	57.7	58.1	2433-2518 bp	1.0	80.3