Hotspot

rpl32-trnL-UAG

SSC · 119681-120976 · 1296 bp

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Pi 0.0077
MarkerSeek score 58.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1296
variable_sites
17
pi
0.0077
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCGTTGAAAGCCTTTTCGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1396-1429 bp 1.0 80.9
rpl32-trnL-UAG_p2 GCGTTGAAAGCCTTTTCGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1397-1430 bp 1.0 80.9
rpl32-trnL-UAG_p3 GCGTTGAAAGCCTTTTCGTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 1406-1439 bp 1.0 79.9
rpl32-trnL-UAG_p4 GGATATTGGACCGCGTTGAA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.3 58.0 1408-1441 bp 1.0 79.5
rpl32-trnL-UAG_p5 GGATATTGGACCGCGTTGAA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.3 58.0 1409-1442 bp 1.0 79.4