Hotspot

trnR-ACG-trnN-GUU

IRb · 113882-114523 · 642 bp

Back to Parinari

Pi 0.00159

MarkerSeek score 61.70

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 642
variable_sites: 2
pi: 0.00159
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	770-783 bp	1.0	79.8
trnR-ACG-trnN-GUU_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	807-820 bp	1.0	79.2
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	811-824 bp	1.0	79.0
trnR-ACG-trnN-GUU_p4	TTTTAGGGTACTCCGGGGAA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	57.7	58.0	824-837 bp	1.0	78.7
trnR-ACG-trnN-GUU_p5	TTTTTAGGGTACTCCGGGGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	57.7	58.0	825-838 bp	1.0	78.7