Hotspot

psaJ-rpl33

LSC · 71365-71862 · 498 bp

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Pi 0.00636

MarkerSeek score 61.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 498
variable_sites: 6
pi: 0.00636
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATCAATCGCTTTTTCCCGGA	GGACATCTTTACCCTTGGCC	45.0	55.0	57.9	58.2	571-580 bp	1.0	78.0
psaJ-rpl33_p2	TCAATCGCTTTTTCCCGGAT	GGACATCTTTACCCTTGGCC	45.0	55.0	57.9	58.2	570-579 bp	1.0	78.0
psaJ-rpl33_p3	GCGTTGACATTCCCGTTTTT	GGACATCTTTACCCTTGGCC	45.0	55.0	57.9	58.2	550-559 bp	1.0	78.0
psaJ-rpl33_p4	TCAATCGCTTTTTCCCGGAT	TTTCGAACACAACCGGTACA	45.0	45.0	57.9	57.7	610-619 bp	1.0	77.4
psaJ-rpl33_p5	TCAATCGCTTTTTCCCGGAT	TGTTTCGAACACAACCGGTA	45.0	45.0	57.9	57.7	612-621 bp	1.0	77.3