Hotspot

petA-psbJ

LSC · 66732-67535 · 804 bp

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Pi 0.00394

MarkerSeek score 47.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 6
pi: 0.00394
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TAACCATAACAGATGCGCCC	GGGTCCATATCAGCAATGCA	50.0	50.0	58.0	58.0	1565-1573 bp	1.0	80.8
petA-psbJ_p2	GGGCGTCAAGTGGTTGATAT	GGGTCCATATCAGCAATGCA	50.0	50.0	58.0	58.0	1542-1550 bp	1.0	80.8
petA-psbJ_p3	CGGATTTGGTCAGGGAGATG	GGGTCCATATCAGCAATGCA	55.0	50.0	58.0	58.0	1438-1446 bp	1.0	80.8
petA-psbJ_p4	TCCTAATGTGGGCGGATTTG	GGGTCCATATCAGCAATGCA	50.0	50.0	57.9	58.0	1450-1458 bp	1.0	80.7
petA-psbJ_p5	CTTGTGATAGGGCCTGTTCC	GGGTCCATATCAGCAATGCA	55.0	50.0	58.0	58.0	1803-1811 bp	1.0	80.5