Hotspot

trnK-UUU-trnQ-UUG

LSC · 4481-5901 · 1421 bp

Back to Parinari

Pi 0.00411

MarkerSeek score 51.00

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1421
variable_sites: 11
pi: 0.00411
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	AAAGCCGAGTACTCTACCGT	TGAATAGAAATGGGGCGTGG	50.0	50.0	58.2	57.9	1536-1547 bp	1.0	78.9
trnK-UUU-trnQ-UUG_p2	AAGCCGAGTACTCTACCGTT	TGAATAGAAATGGGGCGTGG	50.0	50.0	58.2	57.9	1535-1546 bp	1.0	78.9
trnK-UUU-trnQ-UUG_p3	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1478-1489 bp	1.0	78.7
trnK-UUU-trnQ-UUG_p4	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1477-1488 bp	1.0	78.7
trnK-UUU-trnQ-UUG_p5	TACCGTTGAGTTAGCAACCC	TGAATAGAAATGGGGCGTGG	50.0	50.0	57.5	57.9	1522-1533 bp	1.0	76.6