Hotspot

ycf1

SSC · 123458-129151 · 5694 bp

Back to Parietaria

Pi 0.0387

MarkerSeek score 68.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5694
variable_sites: 388
pi: 0.0387
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTAGGATCGGCTTGAAGTCG	CCTTCTTGAATAGCGCGTCT	55.0	50.0	58.2	58.1	268-367 bp	1.0	90.2
ycf1_p2	CTTTGGGCTGGTATCGAGTC	CCTTCTTGAATAGCGCGTCT	55.0	50.0	58.1	58.1	691-694 bp	0.6	72.0
ycf1_p3	TTGTTATCGCCTCCTCTTGC	CCTTCTTGAATAGCGCGTCT	50.0	50.0	58.0	58.1	486 bp	0.2	47.2
ycf1_p4	TGTCTCTTGAACAAACGGGG	CCTTCTTGAATAGCGCGTCT	50.0	50.0	58.0	58.1	531 bp	0.2	47.1
ycf1_p5	GTTATCGCCTCCTCTTGCTC	CCTTCTTGAATAGCGCGTCT	55.0	50.0	58.1	58.1	484 bp	0.2	46.3