Hotspot

clpP

LSC · 70321-72315 · 1995 bp

Back to Parietaria

Pi 0.0225

MarkerSeek score 55.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1995
variable_sites: 79
pi: 0.0225
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATTGGCTGTCGTGTGTTTCT	ATACTCCAACCACCCCATGA	45.0	50.0	58.0	58.0	2839-2887 bp	1.0	86.1
clpP_p2	ATTGGCTGTCGTGTGTTTCT	TGATACTCCAACCACCCCAT	45.0	50.0	58.0	58.0	2841-2889 bp	1.0	86.1
clpP_p3	ATTGGCTGTCGTGTGTTTCT	TCCAACCACCCCATGAATTG	45.0	50.0	58.0	58.1	2835-2883 bp	1.0	85.7
clpP_p4	ATTGGCTGTCGTGTGTTTCT	TACCAATACGCAATGGTCGG	45.0	50.0	58.0	58.1	2412-2441 bp	1.0	84.9
clpP_p5	AGGAGTTAAGGGAAGGGCAT	TACCAATACGCAATGGTCGG	50.0	50.0	58.0	58.1	2795-2823 bp	0.6	65.1