Hotspot

rpl32-trnL-UAG

SSC · 112773-113611 · 839 bp

Back to Parietaria

Pi 0.044

MarkerSeek score 69.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 839
variable_sites: 61
pi: 0.044
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCAGCATTGAAAGCCTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.8	57.9	960-1033 bp	1.0	86.6
rpl32-trnL-UAG_p2	GGCAGCATTGAAAGCCTTTT	GTATTGAATCTCCCCCAGCG	45.0	55.0	57.8	58.1	1628-1707 bp	1.0	85.8
rpl32-trnL-UAG_p3	GGCAGCATTGAAAGCCTTTT	ACAGGAAAAAGCTGCCATCA	45.0	45.0	57.8	58.0	1205-1254 bp	0.6	69.9
rpl32-trnL-UAG_p4	GGCAGCATTGAAAGCCTTTT	CTACCGATTCCACCATAGCG	45.0	55.0	57.8	57.9	943-1016 bp	0.6	68.6
rpl32-trnL-UAG_p5	GGCAGCATTGAAAGCCTTTT	GAATCTCCCCCAGCGAAAAT	45.0	50.0	57.8	57.9	1623 bp	0.2	46.1