Hotspot

petG-trnW-CCA

LSC · 66535-66646 · 112 bp

Back to Parietaria

Pi 0.0625

MarkerSeek score 66.70

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 112
variable_sites: 12
pi: 0.0625
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	AGACGTGGTGATCAATTGGA	CAAAACCCGATGTCGTAGGT	45.0	50.0	56.9	57.9	178-181 bp	1.0	76.8
petG-trnW-CCA_p2	AGACGTGGTGATCAATTGGA	AGAACGTGGGTCTCCAAAAC	45.0	50.0	56.9	58.0	192-195 bp	1.0	76.7
petG-trnW-CCA_p3	CAGACGTGGTGATCAATTGG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	56.8	58.0	193-196 bp	1.0	76.4
petG-trnW-CCA_p4	AGACGTGGTGATCAATTGGA	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	56.9	58.0	214-217 bp	1.0	76.2
petG-trnW-CCA_p5	CAGACGTGGTGATCAATTGG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	56.8	58.0	215-218 bp	1.0	75.9