Hotspot

accD-psaI

LSC · 58912-59612 · 701 bp

Back to Parietaria

Pi 0.0375

MarkerSeek score 61.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 701
variable_sites: 47
pi: 0.0375
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCAAGTAGAAACGTATAAGCCT	TTGCCGGAAATACTAAGCCC	36.4	50.0	54.7	58.0	789-790 bp	0.6	29.4
accD-psaI_p2	TCAAGTAGAAACGTATAAGCCT	AAACCATAGCAATTGCCGGA	36.4	45.0	54.7	58.2	801-802 bp	0.6	29.3
accD-psaI_p3	TCAAGTAGAAACGTATAAGCCT	AACCATAGCAATTGCCGGAA	36.4	45.0	54.7	58.2	800-801 bp	0.6	29.3
accD-psaI_p4	TCAAGTAGAAACGTATAAGCCT	ACCATAGCAATTGCCGGAAA	36.4	45.0	54.7	58.2	799-800 bp	0.6	29.3
accD-psaI_p5	TCAAGTAGAAACGTATAAGCCT	GAAACCATAGCAATTGCCGG	36.4	50.0	54.7	57.8	802-803 bp	0.6	29.3